Cell type-specific manipulation with GFP-dependent Cre recombinase.

Tang J, Rudolph S, Dhande O, Abraira V, Choi S, Lapan S, Drew I, Drokhlyansky E, Huberman A, Regehr W, Cepko C. Cell type-specific manipulation with GFP-dependent Cre recombinase.. Nat Neurosci. 2015;18(9):1334–41.

Abstract

There are many transgenic GFP reporter lines that allow the visualization of specific populations of cells. Using such lines for functional studies requires a method that transforms GFP into a molecule that enables genetic manipulation. We developed a method that exploits GFP for gene manipulation, Cre recombinase dependent on GFP (CRE-DOG), a split component system that uses GFP and its derivatives to directly induce Cre/loxP recombination. Using plasmid electroporation and AAV viral vectors, we delivered CRE-DOG to multiple GFP mouse lines, which led to effective recombination selectively in GFP-labeled cells. Furthermore, CRE-DOG enabled optogenetic control of these neurons. Beyond providing a new set of tools for manipulation of gene expression selectively in GFP(+) cells, we found that GFP can be used to reconstitute the activity of a protein not known to have a modular structure, suggesting that this strategy might be applicable to a wide range of proteins.

Last updated on 03/06/2023