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News & Events

Takesian Lab Opened in November 2017! Ana Castro and Emma He joined the laboratory after giving a fantastic presention at ARO in San Diego! An Early Career Award by NIDCD (March 2018) will fund an exciting project on an unexpected long-range projection from auditory cortex. Vivek Kanumuri, M.D., a...

Microarray Data

As we generate glycan microarray data on our different array platforms and versions, it will be posted publicly here. Click on the corresponding array type or project below. Lectin Quality Assurance / Quality Control Asparagine-Linked Glycans Array Commercial Antibody Data Legacy CFG Microarray Data...

Logos and Guidelines

Resources are provided by the Harvard Otolaryngology– Head and Neck Surgery Communications Office for faculty and staff use only. We welcome you to contact us with questions, suggestions, and/or ideas. Logos To download the complete logos in different formats, select the appropriate insitituion...

Permethylation “Small Scale”

Goal: The purpose of the protocol is to perform small-scale permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever...

Permethylation “Standard Scale”

Goal: The purpose of the protocol is to perform permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever possible, liquid...

Lab Alumni

Shivakumar Vasanth, PhD Title: Investigator, Mass Eye and Ear & Instructor of Ophthalmology, Harvard Medical School Current position: Sr. Staff Scientist / Study director at Covance Google Scholar Reena Gupta, MD Title: Clinical Research Fellow / Cornea Fellow, Mass Eye and Ear Yuming Chen, MD Years...

Cell N-glycan preparation for MS analysis

Goal: To prepare cells for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents are...

Video Lectures

Here is a compiled list of video lectures to help you gain knowledge about glycosciences. Videos from the Programs of Excellence in Glycoscience (PEG) Introduction to Glycobiology (Cardio-PEG LID PEG) (2014) Introduction to Glycobiology (Cardio PEG and LID PEG) (2017) Fundamentals in Glycobiology...

Boston Glycobiology Discussion Group Seminar- Dr. Christina M. Woo

Speaker: Dr. Christina M. Woo (Harvard University) Title: New chemical tools to study O-GlcNAc signaling during immunoactivation Directions: (1) From either Shapiro entrance or Main Enterance use the "Blue Corridor" that connects the different buildings of the BIDMC to get to the Kirstein Building...