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Mice expressing DREADDs and non-DREADD controls were monitored in the Promethion indirect calorimeter, with CNO administered at hour 4 and data plotted in CalR.

C57Bl/6J female mice were monitored for body temperature and metabolic rate during the dark photoperiod without food, with the blue trace representing a fed mouse and the red trace a fasted mouse exhibiting torpor (Hrvatin et al., Nature 2020). ​

Wheel-running activity was continuously recorded over several days, showing hourly running patterns and cumulative wheel counts across alternating light and dark cycles. ​

Alexander S. Banks Beth Israel Deaconess Medical Center Division Of Endocrinology, Diabetes, and Metabolism Center for Life Sciences, 7th floor 330 Brookline Ave Boston, MA 02115 Email: asbanks [at] bidmc.harvard.edu