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About

We are a neuroscience lab focused on the circuitry and plasticity of the central auditory system. Our lab is academically affiliated with Harvard Medical School. We are a part of the Eaton-Peabody Laboratories, the world's largest research center for the study of hearing and deafness, and located on...

Contact

Anne E. Takesian, Ph.D, Principal Investigator anne_takesian@meei.harvard.edu Harvard Medical School Eaton-Peabody Laboratories Massachusetts Eye and Ear 243 Charles Street, Boston MA 02114-3096 Benjamin Glickman, Research Technician benjamin_glickman@meei.harvard.edu Harvard Medical School Eaton...

Glycan Binding Assay with Fusion- or Epitope-tagged Protein

1. Introduction: 1.1.Primary objective is to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on...

2014

December 2014 Harvard Medical School Predicting effective microRNA target sites in mammalian mRNAsVikram Agarwal, PhD, Bartel lab, Whitehead Institute Epigenetic inheritance uncoupled from sequence-specific recruitmentKaushik Ragunathan, PhD, Moazed lab, Harvard Medical School Deciphering the post...

2015

April 2015 Harvard Medical School Exploring unCHARTed territory: Identifying genomic binding sites of noncoding RNAsChris Davis, Kingston Lab, Harvard Medical School/MGH Lipid-mediated regulation of SKN-1/Nrf in response to germ cell ablationMichael Steinbaugh, PhD, Blackwell Lab, Joslin Diabetes...

Serum N-glycan preparation with Rapid PNGaseF for MS analysis

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...