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Goal: To prepare cells for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents are...

Here is a compiled list of video lectures to help you gain knowledge about glycosciences. Videos from the Programs of Excellence in Glycoscience (PEG) Introduction to Glycobiology (Cardio-PEG LID PEG) (2014) Introduction to Glycobiology (Cardio PEG and LID PEG) (2017) Fundamentals in Glycobiology...

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...

Goal: To prepare glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand. Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents...