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Sugar-binding and split domain combinations in repeats-in-toxin adhesins from Vibrio cholerae and Aeromonas veronii mediate cell-surface recognition and hemolytic activities.

Sherik, Mustafa, Robert Eves, Shuaiqi Guo, Cameron J Lloyd, Karl E Klose, and Peter L Davies. 2024. “Sugar-Binding and Split Domain Combinations in Repeats-in-Toxin Adhesins from Vibrio Cholerae and Aeromonas Veronii Mediate Cell-Surface Recognition and Hemolytic Activities.”. MBio 15 (2): e0229123.

Direct Binding Assay + MAWE Protocol

1. Introduction 1.1 Primary objective is to determine the binding specificity of directly labeled Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. Subsequently, remove binding using the MAWE method for future reuse of...

Unlabeled Monoclonal Antibody + MAWE

1. Introduction: 1.1. Primary objective is to determine the binding specificity of a Monoclonal Antibody submitted by investigators, using the printed glycan microarray, and removing with MAWE protocol for reusability. 2. Reference: 2.1. https://research.bidmc.org/ncfg 2.2. Akul Y Mehta, Catherine A...