Annual Society for Glycobiology Meeting
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Glycan Binding Assay with Fusion- or Epitope-tagged Protein
1. Introduction: 1.1.Primary objective is to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on...
2014
December 2014 Harvard Medical School Predicting effective microRNA target sites in mammalian mRNAsVikram Agarwal, PhD, Bartel lab, Whitehead Institute Epigenetic inheritance uncoupled from sequence-specific recruitmentKaushik Ragunathan, PhD, Moazed lab, Harvard Medical School Deciphering the post...
2015
April 2015 Harvard Medical School Exploring unCHARTed territory: Identifying genomic binding sites of noncoding RNAsChris Davis, Kingston Lab, Harvard Medical School/MGH Lipid-mediated regulation of SKN-1/Nrf in response to germ cell ablationMichael Steinbaugh, PhD, Blackwell Lab, Joslin Diabetes...
Serum N-glycan preparation with Rapid PNGaseF for MS analysis
Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...
Collaboration and Service
The NCFG has many ongoing collaborations with various institutions and individuals, including the Resource for Integrated Glycotechnology at the Complex Carbohydrate Research Center, Vector labs, and many academic labs throughout the world. Use the form below for inquiries for service/collaboration...
Glycan Binding Assay on CFG Glycan Microarray Slide with Biotin-Tagged Sample
The following video covers the preparation of the CFG Glycan Microarray slide with Biotin-Tagged protein sample. Click here to view the written protocol. If you have any queries or concerns please feel free to contact us. Credits: Experiment performed by: Richard H. Barnes II Narration by: Richard H...
Cosmc is an X-linked inflammatory bowel disease risk gene that spatially regulates gut microbiota and contributes to sex-specific risk
Kudelka, Matthew, Benjamin Hinrichs, Trevor Darby, Carlos Moreno, Hikaru Nishio, Christopher Cutler, Jianmei Wang, et al. 2016. “Cosmc Is an X-Linked Inflammatory Bowel Disease Risk Gene That Spatially Regulates Gut Microbiota and Contributes to Sex-Specific Risk”. Proc Natl Acad Sci U S A 113 (51): 14787-92.
A novel strategy to enhance angiogenesis in vivo using the small VEGF-binding peptide PR1P
Adini, Avner, Irit Adini, Zai-Long Chi, Ratmir Derda, Amy Birsner, Benjamin Matthews, and Robert D’Amato. 2017. “A Novel Strategy to Enhance Angiogenesis in Vivo Using the Small VEGF-Binding Peptide PR1P”. Angiogenesis 20 (3): 399-408.
Ocular Motor Nerve Development in the Presence and Absence of Extraocular Muscle
Michalak, Suzanne, Mary Whitman, Jong Park, Max Tischfield, Elaine Nguyen, and Elizabeth Engle. 2017. “Ocular Motor Nerve Development in the Presence and Absence of Extraocular Muscle”. Invest Ophthalmol Vis Sci 58 (4): 2388-96.