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A Summary of Services available in the NCFG: Analysis of potential Glycan-binding proteins (GBPs) on various glycan arrays (CFG glycan array, Microbial glycan array, NCFG-derived arrays) Structural analysis of glycans by mass spectrometry and chromatographic techniques. Production of naturally...

Microarray resources currently offered: Name (Click Name for List) Description Availability Reference QC Data Defined Glycan Microarrays Known, defined glycan structures CFG Glycan microarray * Diverse mammalian-type glycan array with N- and O-glycans on NHS slides Yes upon request. CFG Request. CFG...

Please contact us at ncfgservice@bidmc.harvard.edu for any questions.

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Publication: Gao C, Hanes MS, Byrd-Leotis LA, Wei M, Jia N, Kardish RJ, McKitrick TR, Steinhauer DA, Cummings RD. Unique Binding Specificities of Proteins toward Isomeric Asparagine-Linked Glycans. Cell Chem Biol. 2019 Apr 18;26(4):535-547.e4. doi: 10.1016/j.chembiol.2019.01.002. Epub 2019 Feb 7...

Goal: To prepare in-gel glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid...

Microarray Data

Antibody Source Identifier Conc. (ug/ml) Array Type Download Data File HECA-452 BD Pharmingen™ Cat#555946 10 CFG XLSX