Equipment

Analytical

  • UltraFlex II MALDI/TOF Mass Spectrometer (Bruker) – Used for oligosaccharide structural analyses 
  • Fusion Lumos LC-MS Mass Spectrometer (Thermo) – Used for oligosaccharide structural analyses and MS-MS analysis
  • Prominence HPLC Systems with autosampler, fluorescence and UV detectors, and fractions collector (Shimadzu, 4 systems) – Used to isolate and purify glycans for structural analyses and microarray printing
  • ICS-3000 Ion Chromatography (Dionex) – Chromatography system used for glycan composition analysis
  • Vanquish Horizon and Altis Plus (Thermo) - Used for oligosaccharide structural analysis - MLSC Funded

Chemistry

  • CombiFlash Chromatographic System (Teledyne Isco) – Used for separation and fractionation of large scale preparation of glycoconjugates
  • Ozone Generator (Dwyer) – Used to release oligosaccharides from glycosphingolipids for structural analyses, printing as microarrays, and glycan GRIL analyses

Microarrays

  • sciFLEXARRAYER S11 (Scienion) – Non-contact printer used for high precision printing of glycan or protein microarrays
  • sciFLEXARRAYER SX (Scienion) – Non-contact printer used for high precision printing of glycan or protein microarrays - MLSC Funded
  • GenePix 4400A Scanner (Molecular Devices)- Used for scanning fluorescence on microarray slides
  • GenePix 4400A Scanner (Molecular Devices)- Used for scanning fluorescence on microarray slides - MLSC Funded

Microscopy

  • 1X51 Fluorescence Microscope (Olympus) – Used for imaging of cells
  • ImageXpress Pico Automated Cell Imaging System (Molecular Devices) - Used for digital imaging cells in plates and slides.

High Throughput Screening

  • SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices) - Used for measuring UV and fluorescence assays in 96-well or 384-well formats.

  • FLEXMAP 3D (Luminex) - Used for performing bead based assays.

Flow Cytometry

  • Attune Flow Cytometer (Thermo Fisher) - Used for analysis of cell binding by antibodies, lectins, and glycan-binding proteins - MLSC Funded
  • FACSCalibur Flow Cytometer (Becton-Dickenson) – Used for analysis of cell binding by antibodies, lectins, and glycan-binding proteins.

 

* 'MLSC funded' denotes equipment purchased through the Mass Life Science Center Research Infrastructure Funding Program. The Massachusetts Life Sciences Center’s mission is to serve as the hub of the world’s life sciences ecosystem, encourage innovation through investments in good science and business, strengthen and protect Massachusetts’ global leadership position in the life sciences, accelerate the commercialization of promising treatments, therapies, and cures that will improve patient care, create jobs, drive economic development and STEM workforce development.