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  3. Protocols

Protocols

 

Typical Work Flow for Users Making CFG Requests

 

Array Protocols- CFG slides:

  1. Direct Glycan Binding Assay for Fluorescent Labeled Sample
  2. Glycan Binding Assay with Biotin-tagged Sample
  3. Glycan Binding Assay with Unlabeled Monoclonal Antibody
  4. Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum
  5. Glycan Binding Assay with Fusion- or Epitope-tagged Protein
  6. Tertiary Glycan Binding Assay for Unlabeled GBP or Organism Detected with Unlabeled Primary Antibody
  7. Scanning and Analysis

Array Protocols- NCFG slides:

  1. Direct Binding Assay
  2. Biotinylated Sample
  3. Lectin QC
  4. Glycan Binding Assay with Serum/Plasma Sample.

Glycan Analysis Protocols

  1. Cell N-glycan preparation for MS analysis
  2. Serum N-glycan preparation with Rapid PNGaseF for MS analysis
  3. Glycoprotein N-glycan preparation for MS analysis
  4. In-gel glycoprotein N-glycan preparation for MS analysis
  5. O-glycan preparation for MS analysis (all sample types)
  6. Permethylation Small Scale
  7. Permethylation Standard Scale

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  • Resources
    • Microarrays
      • CFG QC Data
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    • Microarrays Pricing
    • Equipment
    • Training
      • Training Videos
        • Glycan Binding Assay on CFG Glycan Microarray Slide with Biotin-Tagged Sample
        • Glycan Microarray Analysis using GenePix Pro
      • Lecture Slides & Book Chapters
      • Common Fund Glycoscience Program Video Interviews
      • Hands-on Training Sessions March 2019
      • Video Lectures
    • Protocols
      • Cell N-glycan preparation for MS analysis
      • Direct Glycan Binding Assay for Fluorescent Labeled Sample
      • Glycan Binding Assay with Biotin-tagged Sample
      • Glycan Binding Assay with Fusion- or Epitope-tagged Protein
      • Glycan Binding Assay with Unlabeled Monoclonal Antibody
      • Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum
      • Glycoprotein N-glycan preparation for MS analysis
      • In-gel glycoprotein N-glycan preparation for MS analysis
      • O-glycan preparation for MS analysis (all sample types)
      • Permethylation “Small Scale”
      • Permethylation “Standard Scale”
      • Serum N-glycan preparation with Rapid PNGaseF for MS analysis
      • Tertiary Glycan Binding Assay for Unlabeled GBP or Organism Detected with Unlabeled Primary Antibody
    • Reagents
    • Bioinformatics and Databases
    • Useful Links

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