Protocols

 

Typical Work Flow for Users Making CFG Requests

 

Array Protocols- CFG slides:

  1. Direct Glycan Binding Assay for Fluorescent Labeled Sample
  2. Glycan Binding Assay with Biotin-tagged Sample
  3. Glycan Binding Assay with Unlabeled Monoclonal Antibody
  4. Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum
  5. Glycan Binding Assay with Fusion- or Epitope-tagged Protein
  6. Tertiary Glycan Binding Assay for Unlabeled GBP or Organism Detected with Unlabeled Primary Antibody
  7. Scanning and Analysis

Array Protocols- NCFG slides:

  1. Direct Binding Assay
  2. Biotinylated Sample
  3. Lectin QC
  4. Glycan Binding Assay with Serum/Plasma Sample.

Glycan Analysis Protocols

  1. Cell N-glycan preparation for MS analysis
  2. Serum N-glycan preparation with Rapid PNGaseF for MS analysis
  3. Glycoprotein N-glycan preparation for MS analysis
  4. In-gel glycoprotein N-glycan preparation for MS analysis
  5. O-glycan preparation for MS analysis (all sample types)
  6. Permethylation Small Scale
  7. Permethylation Standard Scale