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A Summary of Services available in the NCFG: Analysis of potential Glycan-binding proteins (GBPs) on various glycan arrays (CFG glycan array, Microbial glycan array, NCFG-derived arrays) Structural analysis of glycans by mass spectrometry and chromatographic techniques. Production of naturally...

Analytical UltraFlex II MALDI/TOF Mass Spectrometer (Bruker) – Used for oligosaccharide structural analyses Fusion Lumos LC-MS Mass Spectrometer (Thermo) – Used for oligosaccharide structural analyses and MS-MS analysis Prominence HPLC Systems with autosampler, fluorescence and UV detectors, and...

Software tools and resources developed by the NCFG: GLAD - a tool to visualize, analyze and compare glycan microarray data for exploratory data analysis. GlycoGlyph - An open source glycan drawing, visualizing and naming application. Allows you to draw glycans in SNFG format and obtain names in CFG...

The National Center for Functional Glycomics (NCFG) is comprised of a Senior Personnel Center Director Project Leaders Project Coordinator Postdocs, graduate students, and laboratory research staff Administrative Staff There is an External Advisory Committee that helps to guide the center in...

SBIR/STTR The National Institute of General Medical Sciences (NIGMS), the National Eye Institute (NEI), and the National Institute of Dental and Craniofacial Research (NIDCR) have announced SBIR/STTR programs to complement the Common Fund Glycoscience Program. Learn more about these opportunities...

1. Introduction: 1.1.Primary objective is to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on...

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...