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Direct Binding Assay + MAWE Protocol

1. Introduction 1.1 Primary objective is to determine the binding specificity of directly labeled Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. Subsequently, remove binding using the MAWE method for future reuse of...

Glycan Binding Assay with Biotin-tagged Sample + MAWE

1. Introduction: 1.1. Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. Removal of binding with the MAWE method, for future reuse of the slide. 2. References: 2.1. https...

Unlabeled Monoclonal Antibody + MAWE

1. Introduction: 1.1. Primary objective is to determine the binding specificity of a Monoclonal Antibody submitted by investigators, using the printed glycan microarray, and removing with MAWE protocol for reusability. 2. Reference: 2.1. https://research.bidmc.org/ncfg 2.2. Akul Y Mehta, Catherine A...

21] Facts About: Intracellular Protein Glycosylation

By: Richard D. Cummings In the mid 1980s it was strongly believed that all glycosylated proteins arose within the secretory pathway, which generated both secreted and membrane-bound (plasma membrane) glycoproteins. This accepted process was taken to indicate that no glycosylated proteins occurred in...

22] Facts About: X-linked Glyco-Related Genes

By: Richard D. Cummings Among the 900-1,400 genes on the human X chromosome (1, 2) [1], there are many genes important in the metabolism of glycomolecules, including glycosyltransferases, glycohydrolases, transporters, and chaperones. Mutations in several of these X-linked genes, either heritable or...