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The External Advisory Committee (EAC) is comprised of a Committee Chair and 5 additional members. The goal of the EAC is to meet and discuss the Center activities, goals, and directions. An important role of the EAC is to identify key successes, problems, and opportunities of the Center, as well as...

1.Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides...

Note- Click here to view a training video for this protocol. 1. Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www...

1. Introduction: 1.1.Primary objective is to determine the binding specificity of unlabeled Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides...

Goal: The purpose of the protocol is to perform small-scale permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever...

Goal: The purpose of the protocol is to perform permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever possible, liquid...

Shivakumar Vasanth, PhD Title: Investigator, Mass Eye and Ear & Instructor of Ophthalmology, Harvard Medical School Current position: Sr. Staff Scientist / Study director at Covance Google Scholar Reena Gupta, MD Title: Clinical Research Fellow / Cornea Fellow, Mass Eye and Ear Yuming Chen, MD Years...

Goal: To prepare cells for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents are...