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Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al Mol Metabolism 2023)​

Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes. ​

Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​

PARR Instruments Bomb Calorimeter Determines energy content of food or fecal matter FLIR Thermal Imaging Camera Determines thermal radiation

Highly Cited Research from the Core Many discoveries have relied upon careful measurement of body composition and metabolic rates in un-anesthetized, freely moving mice. One metric to determine the impact of a scientific publication is how many times other scientific articles have referred to that...

​ Male C57Bl/6J mice housed at thermoneutrality were administered the β-adrenergic agonist CL 316,243, and changes in oxygen consumption were continuously measured.

C57Bl/6J female mice were monitored for body temperature and metabolic rate during the dark photoperiod without food, with the blue trace representing a fed mouse and the red trace a fasted mouse exhibiting torpor (Hrvatin et al., Nature 2020).

In vivo stable isotope tracing with U-¹³C₆ D-mannitol and U-¹³C₆ D-glucose was conducted to enable ¹³CO₂ monitoring in the Promethion indirect calorimeter, with data plotted using CalR for conventional (blue) and commensal-depleted (red) mice (Vieira et al., in press).

Sable Systems Promethion Indirect Calorimeter (32 cages) Food intake Water intake In-cage body mass measurement Physical activity (infrared beam breaks) Energy expenditure Oxygen consumption (VO2) Carbon dioxide production (VCO2) Respiratory exchange ratio – an indicator of carbohydrate or fat...