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Sable Systems Promethion Indirect Calorimeter (32 cages) Food intake Water intake In-cage body mass measurement Physical activity (infrared beam breaks) Energy expenditure Oxygen consumption (VO2) Carbon dioxide production (VCO2) Respiratory exchange ratio – an indicator of carbohydrate or fat...

Male C57Bl/6J mice (10–14 weeks, JAX) were monitored in the Promethion indirect calorimeter at 30°C, 23°C, and 4°C, tracking body weight and energy expenditure, with data analyzed in CalR. ​

Mice expressing DREADDs and non-DREADD controls were monitored in the Promethion indirect calorimeter, with CNO administered at hour 4 and data plotted in CalR.

C57Bl/6J male mice were monitored in the Promethion indirect calorimeter, and data were analyzed in CalR to compare energy expenditure between T3-treated and vehicle groups (Hochbaum et al., Cell, 2024).

Analysis of diet-induced obesity experiments in male C57Bl/6J mice on a low-fat diet (LFD) or high-fat diet (HFD), demonstrating differences in body weight, energy intake, expenditure, and balance (Banks et al., Nature Metabolism, 2025). ​

Wheel-running activity was continuously recorded over several days, showing hourly running patterns and cumulative wheel counts across alternating light and dark cycles. ​

Physical activity was monitored by infrared beam breaks in the Promethion indirect calorimeter for one hour in either a mouse with ad libitum access to food or shortly after food removal. (Rubio et al., Mol Metabolism, 2023)

EchoMRI 3-in-1 Body Composition Analyzer Measures live mice up to 100g, tissue samples (including Zebrafish) of 10g, and biopsy samples up to 0.1g Measurements include lean mass, fat mass, and water mass 5 minutes per mouse No anesthesia required

Optogenetic stimulation of Agrp neurons in ChR2-expressing mice was performed in the Promethion calorimeter, with food intake, metabolic rate, body temperature, and activity measured during the stimulation period.