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1. Introduction: 1.1.Primary objective is to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on...

The following video covers the preparation of the CFG Glycan Microarray slide with Biotin-Tagged protein sample. Click here to view the written protocol. If you have any queries or concerns please feel free to contact us. Credits: Experiment performed by: Richard H. Barnes II Narration by: Richard H...

December 2014 Harvard Medical School Predicting effective microRNA target sites in mammalian mRNAsVikram Agarwal, PhD, Bartel lab, Whitehead Institute Epigenetic inheritance uncoupled from sequence-specific recruitmentKaushik Ragunathan, PhD, Moazed lab, Harvard Medical School Deciphering the post...

April 2015 Harvard Medical School Exploring unCHARTed territory: Identifying genomic binding sites of noncoding RNAsChris Davis, Kingston Lab, Harvard Medical School/MGH Lipid-mediated regulation of SKN-1/Nrf in response to germ cell ablationMichael Steinbaugh, PhD, Blackwell Lab, Joslin Diabetes...

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...

2018 Spring Scientific Symposium Mechanisms of Inflammation in Health and Disease Tuesday, April 10, 20189:00 am – 5:00 pm Location: Folkman Auditorium, Boston Children's Hospital, 320 Longwood Avenue Scientific Host: Jonathan C. Kagan, PhD Free On-Line Registration is available now 2018 HDDC...

Research Assistant I *Job Posting Job Summary: Under supervision of the principal investigator, conducts a variety of routine experiments using molecular, cell biology and/or biochemistry techniques. Records test results, calculates, transcribes, and analyzes data using basic statistics. The Human...