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Thermogenesis: Cold Temperature Challenge

Male C57Bl/6J mice (10–14 weeks, JAX) were monitored in the Promethion indirect calorimeter at 30°C, 23°C, and 4°C, tracking body weight and energy expenditure, with data analyzed in CalR. ​

Continuous glucose monitoring (CGM) : Telemetry

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al Mol Metabolism 2023)​

Germ-free mice

Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes. ​

Infrared thermal imaging (FLIR)

Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

Body Composition

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​

Photo Gallery

TGF-β Superfamily Conference, Niagra Falls, NY, July 2024. Dr. Nagasuryaprasad Kotikalapudi TGF-β Superfamily Conference, Niagra Falls, NY, July 2024. Dr. Deepti Ramachandran Banks Lab, July 2024. Left to Right: Alex, Evelyn, William, Ellen, Daniel, Prasad, Emily, Deepti

Postdoctoral Fellows

Deepti Ramachandran, Ph.D. Post-Doctoral Fellow dramach1 [at] bidmc.harvard.edu Nagasuryaprasad Kotikalapudi, Ph.D. Post-Doctoral Fellow nkotikal [at] bidmc.harvard.edu

Research Assistants

William Bernard, BASc Research Assistant wbernard [at] bidmc.harvard.edu Marissa Cortopassi, B.S Research Assistant mcortopa [at] bidmc.harvard.edu Gabrielle Paquette, B.A Research Assistant gpaquett [at] bidmc.harvard.edu