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Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum

1. Introduction: 1.1.Primary objective is to determine the binding specificity of Polyclonal Antibodies or Serum submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on the side of the...

Training Videos

The following videos demonstrate commonly used techniques in glycosciences. You can also subscribe to the NCFG YouTube Chanel to get notified about new training videos. Glycan Binding Assay on CFG Glycan Microarray Slide with Biotin-Tagged Sample Glycan Microarray Analysis using GenePix Pro More...

Akul Y Mehta, Ph.D.

Education 2014- Ph.D. in Pharmaceutical Sciences, Medicinal Chemistry from Virginia Commonwealth University. 2007- B. Pharm. in Pharmaceutical Sciences from Mumbai Educational Trust Institute of Pharmacy, University of Mumbai. Background Dr. Mehta obtained his B. Pharmacy from University of Mumbai...

External Advisory Committee

The External Advisory Committee (EAC) is comprised of a Committee Chair and 5 additional members. The goal of the EAC is to meet and discuss the Center activities, goals, and directions. An important role of the EAC is to identify key successes, problems, and opportunities of the Center, as well as...

Direct Glycan Binding Assay for Fluorescent Labeled Sample

1.Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides...

Glycan Binding Assay with Biotin-tagged Sample

Note- Click here to view a training video for this protocol. 1. Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www...

Permethylation “Small Scale”

Goal: The purpose of the protocol is to perform small-scale permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever...

Permethylation “Standard Scale”

Goal: The purpose of the protocol is to perform permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever possible, liquid...