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Lecture Slides & Book Chapters

Lecture Slides Overview of Glycans - by Richard D Cummings | PDF Glycoconjugates - by Richard D Cummings | PowerPoint Show | PDF Book Chapters Discovery and Classification of Glycan-Binding Proteins, Chapter 26 in Essentials of Glycobiology. 2nd edition. Cold Spring Harbor Laboratory Press; 2009...

Glycoprotein N-glycan preparation for MS analysis

Goal: To prepare glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand. Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents...

Glycan Binding Assay with Unlabeled Monoclonal Antibody

1. Introduction: 1.1.Primary objective is to determine the binding specificity of a Monoclonal Antibody submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on the side of the slide...

External Advisory Committee

The External Advisory Committee (EAC) is comprised of a Committee Chair and 5 additional members. The goal of the EAC is to meet and discuss the Center activities, goals, and directions. An important role of the EAC is to identify key successes, problems, and opportunities of the Center, as well as...

Direct Glycan Binding Assay for Fluorescent Labeled Sample

1.Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides...

Glycan Binding Assay with Biotin-tagged Sample

Note- Click here to view a training video for this protocol. 1. Introduction: 1.1.Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) and organisms submitted by investigators, using the printed glycan microarray platform. 2. Reference: 2.1. www...

Microarray Data

As we generate glycan microarray data on our different array platforms and versions, it will be posted publicly here. Click on the corresponding array type or project below. Lectin Quality Assurance / Quality Control Asparagine-Linked Glycans Array Commercial Antibody Data Legacy CFG Microarray Data...

Permethylation “Small Scale”

Goal: The purpose of the protocol is to perform small-scale permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever...

Permethylation “Standard Scale”

Goal: The purpose of the protocol is to perform permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever possible, liquid...