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C57Bl/6J male mice were monitored in the Promethion indirect calorimeter, and data were analyzed in CalR to compare energy expenditure between T3-treated and vehicle groups (Hochbaum et al., Cell, 2024).

Analysis of diet-induced obesity experiments in male C57Bl/6J mice on a low-fat diet (LFD) or high-fat diet (HFD), demonstrating differences in body weight, energy intake, expenditure, and balance (Banks et al., Nature Metabolism, 2025). ​

Wheel-running activity was continuously recorded over several days, showing hourly running patterns and cumulative wheel counts across alternating light and dark cycles. ​

Physical activity was monitored by infrared beam breaks in the Promethion indirect calorimeter for one hour in either a mouse with ad libitum access to food or shortly after food removal. (Rubio et al., Mol Metabolism, 2023)

Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter (Rubio et al., Mol Metabolism, 2023)​

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al., Mol Metabolism, 2023)​

Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes. ​

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​

For any inquiries, please feel free to contact us at: EnergyBalanceCore@bidmc.harvard.edu