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Procedures and Policies

Availability To sign up to use machines, you must be formally trained by the facility staff. Facility notifies users of the webpage, in which you agree to all its policies pertaining to the proper use of the instruments. You cannot train other members in your lab to use the instrumentation. Once you...

About Us

Committed to meet all of the flow cytometry needs for the BIDMC and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting. We are continuously expanding with the newest software and machinery for both...

MOSAIC

CytoFLEX mosaic attaches manually to CytoFLEX LX analyzer. Configuration CytoFLEX mosaic 88 for CytoFLEX LX (88 channels of detection) The pattern of fluorescence channels of detection are: U20, V20, B16, Y12, R10, IR3 for CytoFLEX mosaic 88 + CytoFLEX LX UV SSC on each laser to expand the...

Equipment and Technologies

Fluorescent Activated Cell Sorters ​ Beckman Coulter MoFlo AstriosEQ: A high-speed cell sorter (up to 70,000 cells/second) and analyzer (up to 100,000 cells/second), with four fixed lasers (405nm, 488nm, 561nm, and 640nm). The AstriosEQ has the capacity to acquire 28 parameters simultaneously. In...

MassNano

The flow cytometry core has created a one of its kind nanoparticle characterization facility. Check out all the new equipment to assist with characterizing your particles.

Training

Available by appointment M-F 10am-12pm. Sign-up available through requests tab in iLabs. CytoFLEX LX and Aurora Procedures Only previously trained by staff researchers are allowed to operate and book the analyzers. You are billed according to the time booked. Therefore, please book the time...

John C Tigges, BA, BS

I am the Director/Manager at the Flow Cytometry Core Facility and Center for Extracellular Vesicle Detection at Beth Israel Deaconess Medical Center in Boston, MA (https://research.bidmc.org/flow-cytometry-core/). I have extensive hands-on experience as well as demonstrated expertise in flow...