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Infrared thermal imaging (FLIR)

Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

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Body Temperature Measurement: Telemetry

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter (Rubio et al., Mol Metabolism, 2023)​

Continuous glucose monitoring (CGM) :Telemetry

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al., Mol Metabolism, 2023)​

Germ-free mice

Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes. ​

Body Composition

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​