Annual AAI meeting- Immunology 2017
Visit http://www.immunology2017.org/ Special Guest Society Symposium by the Society for Glycobiology (SfG): Emerging Roles for Glycans in Immunity and Novel Tools to Understand Them
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Lecture Slides & Book Chapters
Lecture Slides Overview of Glycans - by Richard D Cummings | PDF Glycoconjugates - by Richard D Cummings | PowerPoint Show | PDF Book Chapters Discovery and Classification of Glycan-Binding Proteins, Chapter 26 in Essentials of Glycobiology. 2nd edition. Cold Spring Harbor Laboratory Press; 2009...
Glycoprotein N-glycan preparation for MS analysis
Goal: To prepare glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand. Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents...
Glycan Binding Assay with Unlabeled Monoclonal Antibody
1. Introduction: 1.1.Primary objective is to determine the binding specificity of a Monoclonal Antibody submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on the side of the slide...
2] Facts About: The Smallest and the Largest Glycoproteins in Mammals
By: Richard D. Cummings One of the smallest known naturally-occurring glycoprotein in mammals is likely to be CD24, also known as nectadrin. Although it behaves in analyses as a 23-60 kDa glycoprotein, the glycoprotein from mice has only 27 amino acids (human protein has 30 amino acids) (1-3)...
3] Facts About: Pectin – One of the Most Complex Polysaccharides in Nature and its Healing Properties
By: Richard D. Cummings One of the most complex polysaccharides in nature is pectin, found in plant cell walls (1,2). Pectin is a family of galacturonic acid-rich polysaccharides that includes homogalacturonan (HG), rhamnogalacturonan I (RG-I), and the substituted galacturonans rhamnogalacturonan II...
13] Facts About: Bacterial Cellulose
By: Richard D. Cummings While plant cellulose is very famous, many commercial products containing cellulose actually use bacterial cellulose. It is often a part of bacterial biofilms, typically of Gram negative (1). This form of cellulose can be highly purified compared to preparations of plant...
14] Facts About: Keyhole Limpet Hemocyanin (KLH) and its Unusual Glycosylation
By: Richard D. Cummings One of the most unusual glycoproteins and one used commonly in biological research and immunology is KLH, which is derived from the giant keyhole limpet ( Megathura crenulata). The keyhole limpet is a giant aquatic snail (gastropod) with a cone-shaped shell (shell length can...
Consortium for Functional Glycomics Information
The CFG mammalian-type glycan microarray and the Microbial Glycan microarray (MGM) continue to be available for use by request, and we welcome your requests and questions! The requests are reviewed and approved by the CFG Steering Committee (link to paragraph below), led by NCFG Director Dr. Richard...
18] Facts About: Dangerous Carbohydrates?
By: Richard D. Cummings We usually think of polysaccharides as being relatively inert. However, dust explosions in grain elevators and in areas of high density of corn starch or polysaccharide powders occur frequently, as corn starch powder/dust is highly flammable (1). Such dust has tremendous...