Quantitation rates of substrate oxidation in vivo using stable isotope tracing

In vivo stable isotope tracing with U-¹³C₆ D-mannitol and U-¹³C₆ D-glucose was conducted to enable ¹³CO₂ monitoring in the Promethion indirect calorimeter, with data plotted using CalR for conventional (blue) and commensal-depleted (red) mice (Vieira et al., in press).