Nagarajan, Maxwell B, Augusto M Tentori, Wen Cai Zhang, Frank J Slack, and Patrick S Doyle. 2020. “Spatially Resolved and Multiplexed MicroRNA Quantification from Tissue Using Nanoliter Well Arrays.”. Microsystems & Nanoengineering 6: 51.
Abstract
Spatially resolved gene expression patterns are emerging as a key component of medical studies, including companion diagnostics, but technologies for quantification and multiplexing are limited. We present a method to perform spatially resolved and multiplexed microRNA (miRNA) measurements from formalin-fixed, paraffin-embedded (FFPE) tissue. Using nanoliter well arrays to pixelate the tissue section and photopatterned hydrogels to quantify miRNA, we identified differentially expressed miRNAs in tumors from a genetically engineered mouse model for non-small cell lung cancer (K-rasLSL-G12D/+; p53fl/fl). This technology could be used to quantify heterogeneities in tissue samples and lead to informed, biomarker-based diagnostics.