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We use indirect calorimetry systems to measure metabolic rates, physical activity, and food intake in laboratory animals. These components impact energy balance and body weight. These indirect calorimetry systems generate a flood of raw data that requires time-consuming manual manipulation for...

TGF-β Superfamily Conference, Niagra Falls, NY, July 2024. Dr. Nagasuryaprasad Kotikalapudi TGF-β Superfamily Conference, Niagra Falls, NY, July 2024. Dr. Deepti Ramachandran Banks Lab, July 2024. Left to Right: Alex, Evelyn, William, Ellen, Daniel, Prasad, Emily, Deepti

Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al Mol Metabolism 2023)​

Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes. ​

Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​

Male C57Bl/6J mice (10–14 weeks, JAX) were monitored in the Promethion indirect calorimeter at 30°C, 23°C, and 4°C, tracking body weight and energy expenditure, with data analyzed in CalR. ​

EchoMRI 3-in-1 body composition analyzer Measures live mice up to 100g, tissue samples of 10g, and biopsy samples up to 0.1g Measurements include lean mass, fat mass, and water mass 3-5 minutes per mouse No anesthesia required