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Microarray Data

As we generate glycan microarray data on our different array platforms and versions, it will be posted publicly here. Click on the corresponding array type or project below. Lectin Quality Assurance / Quality Control Asparagine-Linked Glycans Array Commercial Antibody Data Legacy CFG Microarray Data...

Permethylation “Small Scale”

Goal: The purpose of the protocol is to perform small-scale permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever...

Permethylation “Standard Scale”

Goal: The purpose of the protocol is to perform permethylation of samples prior to mass spectrometry analysis. Notes: The Preparation of the slurry NaOH/DMSO solution is made fresh every time. Mortar, pestle, and glass tubes are washed with MilliQ water and dry beforehand. Whenever possible, liquid...

Cell N-glycan preparation for MS analysis

Goal: To prepare cells for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents are...

Video Lectures

Here is a compiled list of video lectures to help you gain knowledge about glycosciences. Videos from the Programs of Excellence in Glycoscience (PEG) Introduction to Glycobiology (Cardio-PEG LID PEG) (2014) Introduction to Glycobiology (Cardio PEG and LID PEG) (2017) Fundamentals in Glycobiology...

Array QC Data

We check the quality of all the arrays we produce using lectins and various other reagents. This page contains the QC data for our arrays. Array Type Print Run Tested With Data

Useful Links

Essentials of Glycobiology 4th Edition (Book) (Open Access) NIH Pubmed Harvard Medical School Center for Glycoscience GlyGen KEGG Glycan Congenital Disorders of Glycosylation CAZy Carbohydrate Active Enzymes Predicting O-glycosylation Sites GLYCO3D CarbBank Complex Carbohydrate Research Center at...

Reagents

These reagents are made available through the BIDMC Glycomics Core for fees for shipping and handling only. For the latest reagent shipping and handling fees please see https://www.bidmc.org/research/core-facilities/glycomics-core/fees Please email glycomicscore@bidmc.harvard.edu for all inquiries...

Direct Binding Assay + MAWE Protocol

1. Introduction 1.1 Primary objective is to determine the binding specificity of directly labeled Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. Subsequently, remove binding using the MAWE method for future reuse of...