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Cell N-glycan preparation for MS analysis

Goal: To prepare cells for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents are...

Video Lectures

Here is a compiled list of video lectures to help you gain knowledge about glycosciences. Videos from the Programs of Excellence in Glycoscience (PEG) Introduction to Glycobiology (Cardio-PEG LID PEG) (2014) Introduction to Glycobiology (Cardio PEG and LID PEG) (2017) Fundamentals in Glycobiology...

Healthy Human Serum Anti Carbohydrate Antibodies

Paper The description for how this data was collected can be found in the manuscript titled: Unique repertoire of anti-carbohydrate antibodies in individual human serum Data The following data files can be downloaded GLAD Sessions for 105 serum samples screened against the NCFG microarray for IgG...

Array QC Data

We check the quality of all the arrays we produce using lectins and various other reagents. This page contains the QC data for our arrays. Array Type Print Run Tested With Data

Useful Links

Essentials of Glycobiology 4th Edition (Book) (Open Access) NIH Pubmed Harvard Medical School Center for Glycoscience GlyGen KEGG Glycan Congenital Disorders of Glycosylation CAZy Carbohydrate Active Enzymes Predicting O-glycosylation Sites GLYCO3D CarbBank Complex Carbohydrate Research Center at...

MAL-II Vector Labs

MAL-II ( Maackia amurensis lectin-II); Uniprot: Q7M1M0 Biotinylated Lectins Catalog Number: B-1265 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZA1020 #X1025 CFG, CFG ZD0829 #X1025 QAQC ZF0226 #ZE0521 QAQC CFG ZG0310 #ZF1104 QAQC ZH0928 #ZF1104 QAQC CFG ZJ0916 CFG ZL0809...

SNA Vector Labs

SNA ( Sambucus nigra agglutinin); Uniprot: P33183 Fluorescein Lectins Catalog Number: FL-1301 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZB0106 #X0517 CFG Images ZD0516 #X0517 QAQC Images ZE0111 #X0517 Images ZE0328 #X0517 QAQC ZE1109 #X0517 QAQC ZF1003 #X0517 ZH1111...

GSL-II Vector Labs

GSL-II ( Griffonia simplicifolia lectin II); Uniprot: Q41263 Fluorescein Lectins Catalog Number: FL-1211 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZC0601 #R1122 CFG Images ZH0325 QAQC ZK0321 QAQC ZK0504 QAQC ZL0816 QAQC WOVUS28527 QAQC Biotinylated Lectins Catalog Number...

Reagents

These reagents are made available through the BIDMC Glycomics Core for fees for shipping and handling only. For the latest reagent shipping and handling fees please see https://www.bidmc.org/research/core-facilities/glycomics-core/fees Please email glycomicscore@bidmc.harvard.edu for all inquiries...