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Center Organization

The National Center for Functional Glycomics (NCFG) is comprised of a Senior Personnel Center Director Project Leaders Project Coordinator Postdocs, graduate students, and laboratory research staff Administrative Staff There is an External Advisory Committee that helps to guide the center in...

Glycan Binding Assay with Fusion- or Epitope-tagged Protein

1. Introduction: 1.1.Primary objective is to determine the binding specificity of tagged/fusion Glycan Binding Proteins (GBPs) submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on...

Serum N-glycan preparation with Rapid PNGaseF for MS analysis

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...

Collaboration and Service

The NCFG has many ongoing collaborations with various institutions and individuals, including the Resource for Integrated Glycotechnology at the Complex Carbohydrate Research Center, Vector labs, and many academic labs throughout the world. Use the form below for inquiries for service/collaboration...

Tertiary Binding Assay for Unlabeled sample and Secondary + MAWE Protocol

1. Introduction: 1.1. Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. 2. Reference: 2.1. https://research.bidmc.org/ncfg 2.2. Akul Y Mehta, Catherine A...

ConA Vector Labs

ConA (Concanavalin A); Uniprot: P02866 Fluorescein Lectins Catalog Number: FL-1001 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZA1030 #U1110 CFG ZC0909 #U1110 QAQC Images ZF0124 #U1110 QAQC ZF1025 #U1110 QAQC ZH0312 #U1110+ZG0918 QAQC ZJ0311 #U1110+ZG0918 QAQC ZJ0608 #U1110...

ECL Vector Labs

ECL ( Erythrina cristagalli lectin); Uniprot: P83410 Fluorescein Lectins Catalog Number: FL-1141 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZB0508 #S0512 QAQC CFG Images ZF0712 #S0512 QAQC ZG0526 #ZF0515B QAQC ZH0510 #AF0515B QAQC ZH1202 QAQC ZJ0830 QAQC ZL0930 QAQC...

GNL Vector Labs

GNL ( Galanthus nivalis lectin); Uniprot: P30617 Fluorescein Lectins Catalog Number: FL-1241 Lot Number Root Lot Number QAQC Data Links CFG Data Links Histology Images Z0412 #V1130 QAQC CFG ZF0624 #V1130 QAQC ZH1011 #ZC1003 QAQC ZJ0512 #ZC1003 QAQC ZJ0930 QAQC ZK0322 QAQC WOVUS29916 QAQC...