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August 2013

AUGUST 2013 - The National Institutes of Health (NIH) has awarded a new National Center for Functional Glycomics (NCFG) housed at the Emory University School of Medicine in Atlanta, Georgia, directed by Richard D. Cummings, Ph.D., William Patterson Timmie Professor and Chair of the Department of...

Center Aims/Rationale

The “ National Center for Functional Glycomics” ( NCFG) as a Biomedical Technology Research Center (BTRC) is devoted to the technology of glycan recognition through microarray display and other modes of glycan presentation. This Center is the first of its kind with the central focus being...

Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum

1. Introduction: 1.1.Primary objective is to determine the binding specificity of Polyclonal Antibodies or Serum submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on the side of the...

Glycan Microarray Analysis using GenePix Pro

The following training video demonstrates how to use GenePix Pro to analyze glycan microarray data. If you have any queries or concerns please feel free to contact us. Credits: Experiment performed by: Richard H. Barnes II Narration by: Richard H. Barnes II Produced by: Akul Y. Mehta Introduction by...

O-glycan preparation for MS analysis (all sample types)

Goal: To isolate O-glycan for mass spectrometry analysis. Notes: Glassware and glass tubes are cleaned with Milli Q water and dried beforehand. Reagents are weighted on aluminum foils with utensils clean with Milli Q water beforehand. Whenever possible, liquid reagents are handled with disposable...

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Lectin Quality Assurance / Quality Control

The following page contains the list of commercial lectins tested by the NCFG. Notes: Click on the links to go to the specific pages for the data. On each page you will find data for various lots and for various forms of the lectin (e.g. Fluoresceinated or Biotinylated etc.). On CFG slides, lectins...