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Quanta Magazine - Researchers Read the Sugary ‘Language’ on Cell Surfaces

Glycans, the complex sugars that stud cellular surfaces, are like a language that life uses to mediate vital interactions. Researchers are learning how to read their meaning. Dr. Richard Cummings from the NCFG talks about the role of glycomolecules and the challenges in the field. Read the full...

Resources

A Summary of Services available in the NCFG: Analysis of potential Glycan-binding proteins (GBPs) on various glycan arrays (CFG glycan array, Microbial glycan array, NCFG-derived arrays) Structural analysis of glycans by mass spectrometry and chromatographic techniques. Production of naturally...

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Please contact us at ncfgservice@bidmc.harvard.edu for any questions.

In-gel glycoprotein N-glycan preparation for MS analysis

Goal: To prepare in-gel glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid...