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For Grants and Publications, Please Follow the Templates Below for Proper Acknowledgment of Service Level 1 – Assays and Analyses Performed: “All X, Y, Z, experiments were performed at the Spatial Technologies Unit (STU), Beth Israel Deaconess Medical Center, Harvard Medical School Initiative for...

The 10x Genomics Visium enables hybridization capture of tissue RNA on slides at up to single cell resolution. Visium generates whole transcriptome libraries which are overlapped with high resolution H&E images at a 1-10 cell resolution depending on tissue type. Compatible with fresh frozen and FFPE...

Akoya PhenoCycler-Fusion The PhenoCycler-Fusion enables multidimensional antibody capture in large tissue sections (>1cm2) Up to 65+ antibodies imaged per slide at single cell resolution Create custom antibody panels for frozen tissue sections and FFPE Characterize in-depth cellular populations...

The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in...

The NanoString GeoMx is a versatile platform enabling spatial transcriptomics and/or spatial proteomics (via barcoded antibodies) on selected regions of interest. Select up to 90 regions of interest (ROI) per tissue slide Ability to work seamlessly with FFPE or frozen tissue sections >18,000 genes...

The TMA Grand Master is a fastt, high-capacity and user-friendly tissue microarrayer Allows the precise selection and extraction of specific regions of interest on the donor blocks and their insertion in recipient blocks It enables us to maximize the utility for the spatial technologies available in...

The STU provides fully automated and manual single cell and nucleus sequencing services, including immuno-profiling (VDJ sequencing), cellular indexing of transcriptomes and epitopes (CITE-Seq), and fixed RNA profiling. Single Cell/Nucleus services include: Tissue preparation and dissociation...

The Spatial Technologies Unit has been established in order to provide highly specific but also comprehensive services (from tissue or cells to data). The extensive diversity of cutting-edge instruments enables researchers using the STU to use the optimal technology to a specific problem (and not...

The 10x Genomics Xenium is an in-situ hybridization-based imaging assay that enables high-throughput subcellular mapping of 100s to 1,000s of RNA targets in addition to multiplexed protein on tissue slides. Over 200mm2 imageable area per slide. Compatible with Fresh Frozen and FFPE tissue...