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10x Genomics Single Cell/Nuclei

singlecellgeneexpression

The STU provides fully automated and manual single cell and nucleus sequencing services, including immuno-profiling (VDJ sequencing), cellular indexing of transcriptomes and epitopes (CITE-Seq), and fixed RNA profiling. Single Cell/Nucleus services include:

  • Tissue preparation and dissociation (optional).
  • Single Cell/Nuclei Suspension Preparation (optional).
  • Library preparation and sequencing.
  • Cell Ranger output.
  • Downstream analysis (optional).

For assistance selecting the best assay for your project, schedule a consultation with us, or access the 10x assay selector.

 

Description Species Compatibility Sample Compatibility Workflow Options Throughput
Analysis of TCR/BCR sequencing, combined 5’ gene expression. Human and mouse. Fresh cells or nuclei. Automation. Up to 320,000 cells per run.
Description Species Compatibility Sample Compatibility Workflow Options Throughput
Reverse transcription-based 3′ or 5′ whole transcriptome gene expression. Species non-specific. Fresh cells or nuclei. Sample multiplexing, Automation. Up to 320,000 cells per run.
Description Species Compatibility Sample Compatibility Workflow Options Throughput
5’ gene expression with feature barcoding. Species non-specific. Fresh cells or nuclei. Automation. Up to 320,000 cells per run.
Description Species Compatibility Sample Compatibility Workflow Options Throughput
Probe-based, whole transcriptome 3′ gene expression. Human and mouse. Fresh or fixed cells or nuclei (FFPE). Built-in Multiplexing Up to 1M cells per run.

10xconnect-2326265b

The 10x Genomics Connect addresses the variability of single cell/nucleus preps by automating the complete process from cell/nucleus suspensions to libraries.

  • Generate consistent results: Reduce single cell data variability, generating reproducible and consistent results.
  • Characterize single cells by gene expression or immune receptor profiling.
  • From sample to sequencing-ready libraries in one day
  • Fast turnaround time and maximal consistency to all 10x genomics single cell assays: single nucleus sequencing, single cell sequencing, gene expression + TCR/BCR profiling (VDJ sequencing), and more!

See how the 10x Genomics Connect maximizes single cell robustness and reproducibility https://support.10xgenomics.com/single-cell-gene-expression/index/doc/technical-note-chromium-connect-consistent-automated-single-cell-gene-expression-library-generation

Please follow this link for more information on the instrument: https://www.10xgenomics.com/instruments/chromium-connect