10x Genomics Single Cell/Nuclei

The STU provides fully automated and manual single cell and nucleus sequencing services, including immuno-profiling (VDJ sequencing), cellular indexing of transcriptomes and epitopes (CITE-Seq), and fixed RNA profiling. Single Cell/Nucleus services include:

Tissue preparation and dissociation (optional).
Single Cell/Nuclei Suspension Preparation (optional).
Library preparation and sequencing.
Cell Ranger output.
Downstream analysis (optional).

For assistance selecting the best assay for your project, schedule a consultation with us, or access the 10x assay selector.

Single Cell Immuno-Profiling

Description	Species Compatibility	Sample Compatibility	Workflow Options	Throughput
Analysis of TCR/BCR sequencing, combined 5’ gene expression.	Human and mouse.	Fresh cells or nuclei.	Automation.	Up to 320,000 cells per run.

Single Cell Gene Expression

Description	Species Compatibility	Sample Compatibility	Workflow Options	Throughput
Reverse transcription-based 3′ or 5′ whole transcriptome gene expression.	Species non-specific.	Fresh cells or nuclei.	Sample multiplexing, Automation.	Up to 320,000 cells per run.

Single Cell CITE-Seq

Description	Species Compatibility	Sample Compatibility	Workflow Options	Throughput
5’ gene expression with feature barcoding.	Species non-specific.	Fresh cells or nuclei.	Automation.	Up to 320,000 cells per run.

Fixed RNA Profiling

Description	Species Compatibility	Sample Compatibility	Workflow Options	Throughput
Probe-based, whole transcriptome 3′ gene expression.	Human and mouse.	Fresh or fixed cells or nuclei (FFPE).	Built-in Multiplexing	Up to 1M cells per run.

Chromium Connect

The 10x Genomics Connect addresses the variability of single cell/nucleus preps by automating the complete process from cell/nucleus suspensions to libraries.

Generate consistent results: Reduce single cell data variability, generating reproducible and consistent results.
Characterize single cells by gene expression or immune receptor profiling.
From sample to sequencing-ready libraries in one day
Fast turnaround time and maximal consistency to all 10x genomics single cell assays: single nucleus sequencing, single cell sequencing, gene expression + TCR/BCR profiling (VDJ sequencing), and more!

See how the 10x Genomics Connect maximizes single cell robustness and reproducibility https://support.10xgenomics.com/single-cell-gene-expression/index/doc/technical-note-chromium-connect-consistent-automated-single-cell-gene-expression-library-generation

Please follow this link for more information on the instrument: https://www.10xgenomics.com/instruments/chromium-connect