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Essentials of Glycobiology 4th Edition (Book) (Open Access) NIH Pubmed Harvard Medical School Center for Glycoscience GlyGen KEGG Glycan Congenital Disorders of Glycosylation CAZy Carbohydrate Active Enzymes Predicting O-glycosylation Sites GLYCO3D CarbBank Complex Carbohydrate Research Center at...

12] Facts About: Rare and Unusual Sugars in Nature

By: Richard D. Cummings The definition of a rare sugar is a monosaccharide that is found in small quantities relative to more common sugars in nature, e.g., glucose and N-acetylglucosamine (1-3). There are dozens of rare sugars that are recently gaining attention in regard to nutrition, metabolism...

Tianwei Jia

Dr. Jia earned his Master's in Medicinal Chemistry in 2018 from Tianjin University of Science and Technology, working under the guidance of Prof. Yu Peng. His master’s research concentrated on the synthesis and characterization of glycol-nanoparticles, serving as probes to explore carbohydrate...

Kelsey M Charon

Kelsey received her bachelor's in Biological Engineering with a minor in Chemistry from Wentworth Institute of Technology (August 2023). As a Massachusetts native, she has been living and studying in Boston for the last several years. She has worked on projects with synthetic biology and cell...

Reagents

These reagents are made available through the BIDMC Glycomics Core for fees for shipping and handling only. For the latest reagent shipping and handling fees please see https://www.bidmc.org/research/core-facilities/glycomics-core/fees Please email glycomicscore@bidmc.harvard.edu for all inquiries...

Sugar-binding and split domain combinations in repeats-in-toxin adhesins from Vibrio cholerae and Aeromonas veronii mediate cell-surface recognition and hemolytic activities.

Sherik, Mustafa, Robert Eves, Shuaiqi Guo, Cameron J Lloyd, Karl E Klose, and Peter L Davies. 2024. “Sugar-Binding and Split Domain Combinations in Repeats-in-Toxin Adhesins from Vibrio Cholerae and Aeromonas Veronii Mediate Cell-Surface Recognition and Hemolytic Activities.”. MBio 15 (2): e0229123.

Direct Binding Assay + MAWE Protocol

1. Introduction 1.1 Primary objective is to determine the binding specificity of directly labeled Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. Subsequently, remove binding using the MAWE method for future reuse of...