Molecular Analysis & Assay
Biomarker and Metabolism Research Core
The Biomarker and Metabolism Research Core provides access to XFe 96 Extracellular Flux Analyzer, seahorse technology, as well as additional services that include technical advice in experimental design, data analysis and basic training. The Core also provides affordable access to high performance, electrochemiluminescence immunoassays using MESO QuickPlex SQ 120. This instrument provides unparalleled sensitivity and dynamic range, using low sample volume, and fast, well-organized results. You can perform multiplex biomarker and cytokine assays, test for immunogenicity or toxicity, or we can help you develop your own unique assays. In addition, the Core provides measurement of pyruvate dehydrogenase activity and quantity, Citrate Synthase quantity assay, Cytochrome C assay for mitochondrial research.
For more information, please check out our website.
Director: Xiaowen Liu, PhD
Phone: (617) 667-0706
Email
Flow Cytometry Core
Providing scientists with the ability to rapidly analyze, enumerate, and physically separate cells suspended in fluid, the Flow Cytometry Core offers investigators the newest available software and machinery with both cell sort and analysis capabilities. A combination of highly specialized electronics, micro fluidics, and optics and fluorescence detectors, BIDMC’s flow cytometry instrumentation can simultaneously analyze multiple parameters. We are able to answer technical questions, assist with flow cytometry experiments, analysis of extracellular vesicles, data acquisition, interpretation, and presentation.
For more information, please check out our website.
Director: Peter Weller, MD
Phone: (617) 735-4110
Email
Manager: John Tigges
Phone: (617) 735-4191
Email
Flow Cytometry Technician: Garrett Haskett
Phone: (617) 735-4191
Email
HIV-1 Neutralizing Antibody Core
The HIV-1 Neutralizing Antibody Core provides in vitro HIV-1 neutralizing antibody assay services. The core is GCLP-compliant and is an established resource in the field for assessing the breadth and potency of HIV-1 neutralizing antibodies and for characterizing the neutralization phenotype of HIV-1 isolates.
The core provides services to BIDMC and the external research community. Investigators send monoclonal antibodies or serum samples from clinical cohorts of vaccine studies to the core for in vitro testing using the validated TZM.bl neutralizing antibody assay. The core also has the capability to phenotype primary HIV-1 isolates for sensitivity to broadly neutralizing antibodies.
Director: Mike Seaman, PhD
Phone: 617-735-4449
Email
Core Administrator: Judy Webber
Phone: 617-735-4498
Email
Immunology Technologies Core
The Immunology Technologies Core offers sterile cell sorting and acquisition specifically for sorting live, unfixed and infectious human cells as well as dedicated staff for assistance with instrument operation, data analysis, and panel development.
For more information, please check out our website.
Manager: Michelle Lifton
Phone: (617) 735-4512
Email
Staff Physician: (Chen) Sabrina Tan
Phone:
Email