The Immunology Technologies Core is a departmental core within the Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center. We offer cutting edge flow cytometric, histotechnology and spatial-omics data generation services to clients and collaborators. Our core is directed by Assistant Professor Sizun Jiang, PhD and has two units: Flow Cytometry Unit & Tissue Technologies Unit
Flow Cytometry Unit
The Flow Cytometry Unit (Associate Director: Michelle Lifton) offers sterile cell sorting and acquisition specifically for isolating live, unfixed and infectious human, NHP and murine cells. The unit’s dedicated staff will offer assistance with instrument operation, data analysis, and panel development.
Committed to meeting all of the flow cytometry needs for BIDMC and the external research community, the Flow Cytometry Unit (FCU) offers state-of-the-art instrumentation for routine flow cytometry and cell sorting for both sort and analysis capabilities of up to 18 fluorescent parameters.
The facility provides equipment, and consultation and data analysis services by dedicated personnel to both in-house and outside researchers. Areas of expertise include non-human primate (NHP) and infectious disease research.
The Flow Cytometry Unit is committed to providing the latest software and lasers to better assist peer researchers. The Flow Cytometry Unit facility is affiliated with Harvard University and is supported by the NIH Center for AIDS Research (CFAR).
Tissue Technologies Unit
The Tissue Technologies Unit (Associate Director: Chi Ngai Chan PhD) provides research-focus histology, Immunohistochemistry, In-situ Hybridization (ACDBio RNAscope and Basescope) and Highplex Spatial-omics data generation (Akoya CODEX Fusion, 10X Genomics Visium HD, NanoString GeoMx and CosMx). Our other unique capabilities include BSL-2 NHP tissue processing and cryosectioning, tissue microarray (TMA) construction, tissue clearing, processing of Organ-on-a-chip for spatial assays and multiplexing spatial transcriptomics and proteomics with (PANINI) and IN-DEPTH.
The Tissue Technologies Unit (TTU), part of the Center for Virology and Vaccine Research departmental Immunology Technologies Core in Beth Israel Deaconess Medical Center, offers cutting edge research histotechnology and spatial-omics data generation services to clients and collaborators within the Longwood Medical area and beyond. Our unique capabilities include In-situ Hybridization (ACDBio RNAscope and Basescope) and Highplex Spatial-omics data generation (Akoya CODEX Fusion, Bruker GeoMX and CosMX, 10X Genomics Visium HD), combined Highplex spatial proteomics and transcriptomics on the same slide (IN-DEPTH), BSL-2 NHP tissue processing and cryosectioning, tissue microarray (TMA) construction, tissue clearing, processing of Organ-on-a-chip for spatial assays and multiplexing RNAScope and Protein marker staining (PANINI)
