Serum N-glycan preparation with Rapid PNGaseF for MS analysis
Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...