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BIDMC Energy Balance Core
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Kajimura Lab - 2024
Thermogenesis: Cold Temperature Challenge
Male C57Bl/6J mice (10–14 weeks, JAX) were monitored in the Promethion indirect calorimeter at 30°C, 23°C, and 4°C, tracking body weight and energy expenditure, with data analyzed in CalR.
Continuous glucose monitoring (CGM) : Telemetry
Indirect calorimetry data for one mouse implanted with a glucose and body temperature telemetry probe in the Promethion indirect calorimeter. Food was removed 1 h before start of the dark photoperiod (grey) and mice enter bouts of torpor (Rubio et al Mol Metabolism 2023)
Germ-free mice
Germ-free mice can be examined using isolator cages (bottom). With this approach, we canexamine the metabolic profile of germ-free mice or mice colonized with specific microbes.
Infrared thermal imaging (FLIR)
Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.
Body Composition
Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right.