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Test Page 2023 06 14

Microscopy 1X51 Fluorescence Microscope (Olympus) – Used for imaging of cells ImageXpress Pico Automated Cell Imaging System (Molecular Devices) - Used for digital imaging of cells in plates and slides ImageXpress Pico Automated Cell Imaging System (Molecular Devices) - Used for digital imaging of...

Resources

A Summary of Services available in the NCFG: Analysis of potential Glycan-binding proteins (GBPs) on various glycan arrays (CFG glycan array, Microbial glycan array, NCFG-derived arrays) Structural analysis of glycans by mass spectrometry and chromatographic techniques. Production of naturally...

Contact

Please contact us at ncfgservice@bidmc.harvard.edu for any questions.

In-gel glycoprotein N-glycan preparation for MS analysis

Goal: To prepare in-gel glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand.Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid...

Commercial Antibody Data

Antibody Source Identifier Conc. (ug/ml) Array Type Download Data File HECA-452 BD Pharmingen™ Cat#555946 10 CFG XLSX

HPA Sigma

HPA (Helix pomatia agglutinin) Fluorescein Lectins Catalog Number: Biotinylated Lectins Catalog Number: L6512 Lot Number CFG Data Files QAQC Data Files Image Links SLBP70880 CFG QAQC