Abstract
The periaqueductal gray (PAG) is involved in motor activities, such as movements of the neck, back and hind limbs, cardiovascular regulation, micturition, vocalization, and mating behavior, as well as in nociception control. To accomplish these functions the PAG uses information from other parts of the limbic system, from the lower brainstem, and from the spinal cord. To study the ascending projections from the spinal cord to the PAG, tracer was injected in different parts of the PAG, and the number of retrogradely labeled neurons were counted for each spinal segment. Results show that large segmental differences exist in the number of PAG projecting neurons throughout the length of the spinal cord and that different parts of the spinal cord project to specific areas in the PAG.