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The NCFG operates under 3 TR&D projects: Project 1 – Expansion of Glycan Microarray Technology Project 2 – Shotgun Glycomics and Functional Recognition Project 3 – Glycan Display and Recognition TR&D project goals will focus on 5 main areas and the projects integrates and synergizes with each other...

Goal: To prepare glycoproteins for N-glycan isolation and analysis by mass spectrometry. Notes: Glassware and glass tubes are cleaned with MilliQ water and dried beforehand. Reagents are weighted on aluminum foil with utensils cleaned with MilliQ water beforehand. Whenever possible, liquid reagents...

1. Introduction: 1.1.Primary objective is to determine the binding specificity of a Monoclonal Antibody submitted by investigators, using the printed glycan microarray. 2. Reference: 2.1. www.functionalglycomics.org 3. Materials needed: 3.1.Glycan printed slides , printed on the side of the slide...

The CFG mammalian-type glycan microarray and the Microbial Glycan microarray (MGM) continue to be available for use by request, and we welcome your requests and questions! The requests are reviewed and approved by the CFG Steering Committee (link to paragraph below), led by NCFG Director Dr. Richard...

1. Introduction 1.1 Primary objective is to determine the binding specificity of tagged Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. Subsequently, remove the binding using the MAWE protocol for future reuse of the...

Name: AAL- Aleuria aurantia lectin; Uniprot: P18891 Fluorescein Lectins Catalog Number: FL-1391 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZA1120 #W0730 CFG Images ZE1101 #W0730 QAQC ZG0316 #Z1202 QAQC ZH0914 #Z1202 QAQC ZJ0830 QAQC ZK0321 QAQC ZK0522 QAQC WO40414 QAQC...

PHA-E ( Phaseolus vulgaris Erythroagglutinin); Uniprot: P05088 Fluorescein Lectins Catalog Number: FL-1121 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images Y0530 #61027 QAQC CFG ZG1012 QAQC ZH0820 QAQC ZJ0830 QAQC ZK0317 QAQC WO42965 QAQC Biotinylated Lectins Catalog Number: B...

PNA (Peanut agglutinin); Uniprot: P02872 Fluorescein Lectins Catalog Number: FL-1071 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZA0227 #U0125 QAQC CFG Images ZF1023 QAQC ZH0616 QAQC ZJ0712 QAQC ZH1221 QAQC ZJ0826 QAQC ZJ0712 QAQC ZK0224 QAQC ZK0718-10 QAQC ZK0718-5 QAQC...

WFA ( Wisteria floribunda agglutinin); Uniprot: A0A218PFP3 Fluorescein Lectins Catalog Number: FL-1351 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZB0107 #Y0510 CFG Images ZE1101 #Y0510 QAQC ZF0315 #Y0510 QAQC ZG1113 #ZG0526 QAQC ZH0824 #ZG0720/ZG0914 QAQC ZK0901 QAQC...

Array Protocols- Reusing Glycan Microarray Slides Using MAWE Direct Binding Assay + MAWE Protocol Glycan Binding Assay with Biotin-tagged Sample + MAWE Unlabeled Monoclonal Antibody + MAWE Glycan Binding Assay with tagged Sample on CFG Slides + MAWE Tertiary Binding Assay for Unlabeled sample and...