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The following video covers the preparation of the CFG Glycan Microarray slide with Biotin-Tagged protein sample. Click here to view the written protocol. If you have any queries or concerns please feel free to contact us. Credits: Experiment performed by: Richard H. Barnes II Narration by: Richard H...

Goal: To prepare serum for N-glycan isolation by Rapid PNGaseF and analysis by mass spectrometry. Notes: Solvents are HPLC grade or higher. Protocol: Lyophilize 5 to 10 µl of serum (achieve in less than an hour) in a 500 µl tube or in a 200 µl PCR tube if using a PCR thermocycler for buffer and...

The NCFG has many ongoing collaborations with various institutions and individuals, including the Resource for Integrated Glycotechnology at the Complex Carbohydrate Research Center, Vector labs, and many academic labs throughout the world. Use the form below for inquiries for service/collaboration...

The Legacy CFG Microarray Data can be found at: https://functionalglycomics.org/glycan-array

RCA-I ( Ricinus communis agglutinin, RCA120); Uniprot: P06750 Fluorescein Lectins Catalog Number: FL-1081 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZC0705 #Z0607 QAQC CFG ZD1204 #Z0607 QAQC Images ZF0529 #Z0607 QAQC ZF1107X #Z1028 QAQC ZH0302 #ZA0804 QAQC ZH0824 #ZG0930...

MAL-I ( Maackia amurensis lectin-I); Uniprot: P0DKL3 Fluorescein Lectins Catalog Number: FL-1311 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZB0210 #U0619 QAQC CFG Images ZD0222 #U0619 CFG Images ZF0815 #U0619 QAQC ZH1027 #ZF0206 QAQC ZJ0607 #ZF0206 QAQC ZJ0916 QAQC ZL0620...

ECL ( Erythrina cristagalli lectin); Uniprot: P83410 Fluorescein Lectins Catalog Number: FL-1141 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZB0508 #S0512 QAQC CFG Images ZF0712 #S0512 QAQC ZG0526 #ZF0515B QAQC ZH0510 #AF0515B QAQC ZH1202 QAQC ZJ0830 QAQC ZL0930 QAQC...

ConA (Concanavalin A); Uniprot: P02866 Fluorescein Lectins Catalog Number: FL-1001 Lot Number Root Lot # QAQC Data Links CFG Data Links Histology Images ZA1030 #U1110 CFG ZC0909 #U1110 QAQC Images ZF0124 #U1110 QAQC ZF1025 #U1110 QAQC ZH0312 #U1110+ZG0918 QAQC ZJ0311 #U1110+ZG0918 QAQC ZJ0608 #U1110...

GNL ( Galanthus nivalis lectin); Uniprot: P30617 Fluorescein Lectins Catalog Number: FL-1241 Lot Number Root Lot Number QAQC Data Links CFG Data Links Histology Images Z0412 #V1130 QAQC CFG ZF0624 #V1130 QAQC ZH1011 #ZC1003 QAQC ZJ0512 #ZC1003 QAQC ZJ0930 QAQC ZK0322 QAQC WOVUS29916 QAQC...

1. Introduction: 1.1. Primary objective is to determine the binding specificity of Glycan Binding Proteins (GBPs) submitted by investigators. The primary array platform is the CFG printed defined glycan microarray. 2. Reference: 2.1. https://research.bidmc.org/ncfg 2.2. Akul Y Mehta, Catherine A...