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Thermal imaging was performed using FLIR on juvenile mice placed in a sterile 6-well culture dish to visualize body temperature distribution.

Body composition was measured using the Echo MRI 3-in-1 system and plotted in CalR, with fat, lean, and total mass shown on the left and corresponding percentage composition on the right. ​

Male C57Bl/6J mice (10–14 weeks, JAX) were monitored in the Promethion indirect calorimeter at 30°C, 23°C, and 4°C, tracking body weight and energy expenditure, with data analyzed in CalR. ​

EchoMRI 3-in-1 body composition analyzer Measures live mice up to 100g, tissue samples of 10g, and biopsy samples up to 0.1g Measurements include lean mass, fat mass, and water mass 3-5 minutes per mouse No anesthesia required