Our facility is equipped with a comprehensive suite of modern instruments supporting the full pipeline—from protein expression and purification to crystallography, biophysical characterization, and advanced structural determination:
Protein Expression & Cell Culture
- Eppendorf S41i CO₂ Shaker Incubators (x2) — optimized for suspension CHO, HEK-293, Sf9, and Tni cell expression.
- Infors HT Incubators (x2) — enable large-scale insect and mammalian cell culture (up to 20 L).
Protein Purification & Sample Preparation
- Bio-Rad NGC Chromatography Systems (x2) — configured with multiple columns for affinity, ion-exchange, SEC, and polishing workflows.
- TTP Labtech Mosquito Nanoliter Pipetting Robot — precision low-volume crystallization setup and screening.
- Matrix Hydra DT — high-throughput 96-well liquid handling for assay and crystallization plate preparation.
X-ray Crystallography
- Rigaku Micromax-007 X-ray Generator
- R-Axis HTC Detector
- Osmic VariMax HF Confocal Optics
- X-Stream 2000 Cryogenic System — enabling data collection at 100K.
- Leica Z6 Stereo Microscope with Polarizer
- Two Olympus Microscopes — for crystal inspection, imaging, and documentation.
- Two Temperature-Controlled Incubators (4°C – 25°C) — for crystallization screens and optimization.
- Spot Flex 15.2 (64 MP) Shifting Pixel Digital Camera
- Art Robbins Cryscam — automated high-throughput crystallization plate imaging.
Biophysical and Analytical Characterization
- Refeyn Two-MP Mass Photometer — rapid, low-sample mass measurement and oligomerization assessment.
- MicroCal ITC — thermodynamic and binding interaction analysis.
- Dynamic Light Scattering (DLS) and Multi-Angle Light Scattering (MALS)
- NanoTemper Tycho — thermal stability profiling and buffer optimization.
- Bio-Rad CFX Connect qPCR System — supporting thermal shift assays and melt-curve analysis.
- Peak Scientific Nitrogen Generator — uninterrupted high-purity nitrogen supply.
Advanced Structural Biology Through Collaborative Cryo-EM
In addition to our on-site capabilities, we collaborate closely with Harvard Medical School, the Massachusetts Institute of Technology, and Boston University Cryo-EM facilities to provide access to high-resolution structure determination by cryo-electron microscopy. These partnerships allow investigators to pursue single-particle analysis, tomography, and complex molecular assemblies—supported by expert guidance from our facility throughout sample preparation, grid optimization, data collection, and model building.