Our facility offers comprehensive, end-to-end support for protein production, structural biology, and biophysical characterization—whether your goal is basic research, drug discovery, or translational development.
Molecular Biology & Cloning
- Custom cloning of your gene of interest into a wide range of bacterial, insect, and mammalian expression vectors.
- Access to hundreds of expression vectors and gene templates on-site to accelerate project timelines.
- Gene synthesis and insertion into virtually any commercial or custom vector—including complex, multi-gene, and tagged constructs.
- Production of endotoxin-free plasmids and bacmids for high-efficiency expression across multiple systems.
Protein Expression & Purification
- Small- and large-scale protein expression in rich or minimal media for bacteria, insect, and mammalian cells.
- Purification of recombinant proteins for crystallography, cryo-EM, NMR, functional assays, and therapeutic development.
- Consultation and troubleshooting on expression optimization, construct design, and host system selection.
Structural Biology Preparation & Analysis
- Sample preparation for X-ray crystallography, NMR, cryo-electron microscopy, and biophysical assays.
- Optimization of crystallization or cryo-EM conditions for challenging targets.
- X-ray data collection at ambient temperature and 100K using on-site instrumentation.
- Synchrotron data collection coordination and support for beamlines at BNL (NSLS-II), APS, Diamond, ESRF, and EMBL.
- Expertise in phasing strategies including molecular replacement, SAD/MAD, and multiwavelength approaches.
- Structure solution, refinement, and in-depth structural analysis with publication-quality figures.
Biophysical and Functional Support
- Protein stability, oligomeric state, and interaction assessment using a range of analytical tools.
- Access to commonly used proteases, polymerases, kinases, phosphatases, and other enzymes maintained in stocked inventory—available upon request.
Collaborations
- Active collaboration with the NMR Core Facilities at Harvard Medical School for macromolecular and small-molecule characterization.
- Collaborative pathways for cryo-EM structure determination through Harvard Medical School, MIT, and Boston University Cryo-EM centers.